I have 2 sequencing results at attachment. They are different PCR products and was used same primers. For 2 samples after PCR purification with exosapit, below protocol was used:
Big Dye v3.1: 2 ul
Buffer: 1 ul
primer: 1 ul from 3.2 pmol
pcr: 1 ul
water: 5 ul
Sephadex was used.
Sequence machine is 3130. Pop7 and buffers are fresh.
Samples were checked with agarose gel electr. after PCR, and their bands are good and one.
But my sequence results are not very good.
What is your recommendations about this results?
Thanks for your help