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looking for DNA fiber analysis protocol for S.pombe.


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#1 parharn

parharn

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Posted 07 October 2014 - 02:00 AM

Hi,

I am trying to do DNA fiber analysis (combing) on S. pombe and I could have find some protocols and try them, but unfortunately none are working. However there were not describing S. pombe DNA preparation but Human cell lines. So I modified them a bit hoping that it will work for pombe but it didn't. So I was wondering if any one knows a well explained protocol to do DNA fiber analysis on S. pombe? I really appreciate that.

 

Thanks!






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