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# Dilute to a working concentration

dilution

Best Answer mdfenko, 06 October 2014 - 03:44 AM

Hi,

not sure of it is the right place but..

For some reason I just cannot wrap my head around it.

I've calculated that I need 0,15 ul of a reagent to be added in 150 ul of DMEM to get a c2=0,1 ug/ml.

(0,1 ug/ml x 1,5 ml) / 1000 ug/ml = 0,00015 ml = 0,15 ul.

c2                 v2           c1                   v1

However, I want to dilute this to a working concentration, e.g. 1:100 to get v1 to 15 ul.

How to practically do this?

And will it not affect the end concentration (c2)?

Thanks

add 1 ul of the stock to 99 ul of diluent.

the only variable affected by changing c1 is v1, which is what you wanted.

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1 reply to this topic

### #1 dendrite

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Posted 06 October 2014 - 02:22 AM

Hi,

not sure of it is the right place but..

For some reason I just cannot wrap my head around it.

I've calculated that I need 0,15 ul of a reagent to be added in 150 ul of DMEM to get a c2=0,1 ug/ml.

(0,1 ug/ml x 1,5 ml) / 1000 ug/ml = 0,00015 ml = 0,15 ul.

c2                 v2           c1                   v1

However, I want to dilute this to a working concentration, e.g. 1:100 to get v1 to 15 ul.

How to practically do this?

And will it not affect the end concentration (c2)?

Thanks

### #2 mdfenko

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Posted 06 October 2014 - 03:44 AM   Best Answer

Hi,

not sure of it is the right place but..

For some reason I just cannot wrap my head around it.

I've calculated that I need 0,15 ul of a reagent to be added in 150 ul of DMEM to get a c2=0,1 ug/ml.

(0,1 ug/ml x 1,5 ml) / 1000 ug/ml = 0,00015 ml = 0,15 ul.

c2                 v2           c1                   v1

However, I want to dilute this to a working concentration, e.g. 1:100 to get v1 to 15 ul.

How to practically do this?

And will it not affect the end concentration (c2)?

Thanks

add 1 ul of the stock to 99 ul of diluent.

the only variable affected by changing c1 is v1, which is what you wanted.

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