Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

ldh-L promoter primers

  • Please log in to reply
1 reply to this topic

#1 tomgar



  • Active Members
  • Pip
  • 13 posts

Posted 12 July 2004 - 01:36 AM

I am biotechnology student from Poland. I work with
Lb.plantarum in my lab. I make master thesis.I have to
construct vector with expressed gfp gene. The problem is to
find strong promoter. I heard it has strong promoter
. So there is my question: I would like use chromosomal
DNA to amplify ldhl promoter.Where can I find sequence of ldhl
gene promoter in GenBank, I would like to design primers to
amplify this promoter from chromosomal DNA of my Lb.plantarum
NCFB1193 . HOW to do it? is it possible ? Maybe there is another strong
promoter which i could use?Please, give me some tips, I'll be
very grateful.

#2 pcrman



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts

Posted 12 July 2004 - 10:34 PM

Are you trying to amplify ldhl promoter from plantarum genome? I searched genbank and only got two sequences and no promoter region has been defined in these sequence. you may want to check the literature to see what others have used.

if you want to get the promoter sequnce from other genomes, it may be easy to search.

btw, why don't you just buy a vector with gfp gene driven by some types of promoters such as SV40, lac, etc. Some vectors even have enhancer which guarantee high level of transcription.

1: AB177763
Lactobacillus plantarum l-ldhL gene for L-lactate dehydrogenase, complete cds

2: X70926
L.plantarum gene for l-lactate dehydrogenase

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.