I have cloned EGFP under the control pspac promoter and lac operon system and made L. monocytogenes mutant with this. I used IPTG, an analog to lactose, however I could not get EGFP expressed in the culture.
Posted 17 October 2014 - 03:29 PM
I'm not familiar with a pspac promoter or L. monocytogenes, but assuming that expression of your plasmid in this organism is a well documented system then my bet is that there is a problem in your gene sequence. Have you had your plasmid sequenced?