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EGFP expression


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#1 Suvash

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Posted 30 September 2014 - 06:20 PM

I have cloned EGFP under the control pspac promoter and lac operon system and made L. monocytogenes mutant with this.  I used IPTG, an analog to lactose, however I could not get EGFP expressed in the culture.



#2 labtastic

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Posted 17 October 2014 - 03:29 PM

I'm not familiar with a pspac promoter or L. monocytogenes, but assuming that expression of your plasmid in this organism is a well documented system then my bet is that there is a problem in your gene sequence. Have you had your plasmid sequenced?






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