I did the experiment a number of times buy am still not able to obtain the expected result. I would like to seek help from everyone. The protocol as follows:
1. Dissolve 1%maltodextrin and 1%CMC (carboxymethyl cellulose) separately in sterile distilled water.
2. Centrifuge probiotic culture and wash with PBS.
3. Dissolve pellet with 1% maltodextrin and follow by CMC, stir using magnetic stirrer for 5 minutes.
4. Perform acidity test for the mixture.
Result obtained: survivability of control sample is higher than malto+CMC encapsulated sample at pH1.5 for 90min.
May I know which step went wrong? Or, is it a MUST to perform spray drying process in order to obtained the encapsulated cells?