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Can anyone help me to check on the protocol of probiotic microencapsulation?

microbiology

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#1 Celz

Celz

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Posted 28 September 2014 - 05:22 PM

I did the experiment a number of times buy am still not able to obtain the expected result. I would like to seek help from everyone. The protocol as follows:

 

1. Dissolve 1%maltodextrin and 1%CMC (carboxymethyl cellulose) separately in sterile distilled water. 

2. Centrifuge probiotic culture and wash with PBS. 

3. Dissolve pellet with 1% maltodextrin and follow by CMC, stir using magnetic stirrer for 5 minutes. 

4. Perform acidity test for the mixture. 

 

Result obtained: survivability of control sample is higher than malto+CMC encapsulated sample at pH1.5 for 90min. 

 

May I know which step went wrong? Or, is it a MUST to perform spray drying process in order to obtained the encapsulated cells? 

 

Thanks.

 

 







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