I hope somebody can help me.
We transfected cells with a construct. After antibiotic selection we obtained many cell clones.
We made a real-time PCR and chose the best clones for further analysis.
Then we made a Western blot... What we see is surprising: two clones have a small protein band size (around 50 kDa), and all the rest bigger (around 80 KDa). The clones with the small size protein react to our experimental conditions in a differnet way as all others.
What happened? posttranslational modification? alternative splicing???