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Exon junction exclusion from primers for Real Time PCR

Exon junction primers

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#1 Poorti

Poorti

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Posted 25 September 2014 - 11:10 PM

Hi I am working on a gene which has a single exon. So, for mRNA detection by real time PCR, I cannot include exon-exon junction in my primer pair. I wanted to know if, I should design reference genes' primer pairs from a single exon as well. Thanks.





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