Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Exon junction exclusion from primers for Real Time PCR

Exon junction primers

  • Please log in to reply
No replies to this topic

#1 Poorti



  • Members
  • Pip
  • 4 posts

Posted 25 September 2014 - 11:10 PM

Hi I am working on a gene which has a single exon. So, for mRNA detection by real time PCR, I cannot include exon-exon junction in my primer pair. I wanted to know if, I should design reference genes' primer pairs from a single exon as well. Thanks.

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.