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Na butyrate in lysis buffer for Ac Histone Western Blotting

Na butyrate use Ac His detection

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#1 Poorti

Poorti

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Posted 25 September 2014 - 10:29 PM

I want to detect Acetylated Histone by Western Blotting. I was wondering, whether I need to add Na butyrate in my lysis buffer or not, since, most of the protocols don't mention using Na butyrate. Please suggest if I should also proceed without adding Na butyrate. All suggestions would be highly appreciated. Thanks.




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