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Spotted DAB staining!

IHC H&E Tissue microarray

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#1 Ambinlab

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Posted 25 September 2014 - 08:57 PM

Hi all,

 

I have stained a slide of normal human tissues with a specific antibody against my protein of interest. What I observed is that in some tissues like lung and kidney, there are brown spots whereas in liver it is more like cytoplasmic brown staining. I have attached a photo of lung, I would appreciate any comment, since I have never done this before and do not have any idea as to what it might be.

 

Thanks a lot.

 

 

PS: Initially I thought it might refer to localization in one of the organelles, but in some cells there are three or four of those spots,...what can it be?

 

Attached Thumbnails

  • Screen Shot 2014-09-25 at 10.55.17 PM.png
  • Screen Shot 2014-09-25 at 10.54.49 PM.png


#2 Ambinlab

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Posted 28 September 2014 - 04:12 PM

Hi All,

 

sorry I am still stuck in this issue. Any ideas?







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