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A worried PhD student - How much is required for my thesis?

Thesis Writing PhD ChIPseq Genomics NGS

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#1 j47027

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Posted 25 September 2014 - 11:06 AM

Hi guys,

 

I have just gone into my final year of funding for my PhD (4th year). I am worried about finishing on time. I know a lot of people don't finish on time but due to my boss being absent for multiple 3 month periods for personal reasons I have been going through experiments with little guidance. If I go into my 5th year I have to pay my own fees and support myself until i finish. I am getting to the point were everything is getting on top of me that I can't seem to get a whole lot of work done.

 

Without going into detail - My PhD project involves ChIPseq of 3 centomere components both in async cells and defined cell cycle stages. Due to problems in establishing cell lines and antibodies that are/were not chip grade I haven't actually carried out any chipseq experiments yet. What I have done though is taught myself bioinformatics analysis from scratch (nearly 2 years of work) and processed a lot of our collaborators chipseq experiments which are carried out on other centromere components which have shown a number of novel findings. My boss is absent again so i have organised to fly to my collaborators lab for 8 weeks to finish of more bioinformatics and carry out two chipseq experiments. Ideally my PhD thesis chapters would be..

1. Introduction

2. Materials Methods

3. Results - Bioinformatics Analysis of collaborators experiments

4. Results - ChIPseq of centromere components Async Cells

5. Results - ChIPseq of centromere components Cell cycle stages

6. Disucssion...etc

 

My colleague is establishing cell cycle synchronisation parameters at the moment and should be optimised by the time im back (christmas). Chromatin preparations are optimised and qPCR conditions are also optimised - the company we are sequencing with are carrying out adapter ligations so essentially all we have to send is the chiped DNA. I have for sure 1 antibody that is working in ChIP and 1 that hasn't been tested right through to qPCR but Western analysis looks promising. The third antibody may have to be discarded but I'm also making an antibody to replace this.

 

My questions are - How many ChIPseq experiments would you need to have enough for a PhD thesis? Would three chipseq experiments in async cells be enough to finish? What happens if some of these experiments fail? How can I motivate myself to get this done?!

 

Thanks for taking time to read this - I can give you more information

 



#2 Tabaluga

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Posted 25 September 2014 - 12:05 PM

Hi, I can't answer your questions about how much is enough for the thesis, and I hope that someone will be able to answer this soon, but I can say something about this question: "How can I motivate myself to get this done?!"

You are not alone with this. A lot of us have trouble during their thesis, advisors who don't care or are always absent, failing experiments etc.

My own thesis (not PhD) was one long struggle or rather one long succession of failing experiments and worries, so I know how you feel. My motivation also dropped during times, but I kept going because I still had (and have) the final aim of getting that title and publish something, and to see that all the effort was not done in waste.

When your motivation drops, just think of how it will be like when you finally get that PhD you worked so hard for, and think about how you've come a long way and now it's just about the "last steps" to be still taken...


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 


#3 j47027

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Posted 25 September 2014 - 12:15 PM

Hi - Thank you for taking the time to answer

 

I try to think like that as much as I can. One good thing to come is that I am getting a publication soon. I won't be first author but I will have my own figures so I am happy with that. 

 

Its just the pressure is on now and part of me just wants to leave and never go back but I know Ill regret that. It's a horrible feeling giving pretty much the same presentation to my department every time (we present quite often). Hopefully I can get some more experiments done on my trip away.



#4 A h m a d

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Posted 25 September 2014 - 12:23 PM

Hello.

 

You are definitely in the same boat as the other PhD students :)

 

Don't worry, it is stressful :) Just be happy that you are nearly done!

 

Ahmad







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