Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

Harvesting cells

Started by Biochem_newbie, Sep 24 2014 06:37 AM

Please log in to reply

3 replies to this topic

#1 Biochem_newbie

Enthusiast

Active Members
32 posts

0
Neutral

Posted 24 September 2014 - 06:37 AM

Hi there,

I was wondering if someone can confirm if this is right method or wrong method for harvesting cells.

For 293s, resuspend the cells with cold PBS, spin down, discard the sup, and add lysis buffer with protease inhibitor dissolved.

Is this correct?

Back to top

#2 Tabaluga

Making glass out of shards

Active Members
427 posts

51
Excellent

Posted 24 September 2014 - 07:02 AM

So you wanna harvest cells - but what for ?

Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

Back to top

#3 Biochem_newbie

Enthusiast

Active Members
32 posts

0
Neutral

Posted 24 September 2014 - 07:45 AM

Apologies for the vagueness.

I would like to prepare over-expressed samples in 293 for WB

Back to top

#4 bob1

Thelymitra pulchella

Global Moderators
5,908 posts

426
Excellent

Posted 25 September 2014 - 08:09 PM

In that case, yes, that is one method of preparing lysates. There are a number of other options as well, but basically they all involve washing the cells in a buffer then lysing.

Back to top

Back to Tissue and Cell Culture · Next Unread Topic →

Google Sign in options

Harvesting cells

#1 Biochem_newbie

#2 Tabaluga

#3 Biochem_newbie

#4 bob1

Sign In