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Transfection media change


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#1 Biochem_newbie

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Posted 17 September 2014 - 08:17 AM

Hi there,

I have always been hesitant of media change after transfection, especially in 293s because of the cells lifting up afterwards.

I was wondering if anyone had any tips, tricks, and/or any suggestions in order to avoid cells lifting up.

Thanks

#2 Tabaluga

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Posted 17 September 2014 - 10:49 AM

Hi, maybe some of this might help:

 

https://www.research...try_experiments

http://www.protocol-...osts/20486.html


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 





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