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Clone Selection on Jurkat Cells

limiting dilution clone selection Jurkat G418

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#1 RomanSh

RomanSh

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Posted 14 September 2014 - 11:51 PM

Hello

 

I have transfected Jurkat cells with a plasmid contating my gene and resistance to G418.

I want to perform limiting dilution in order to obtain a cell population from a single clone.

 

Does somebody has a protocol, advice or tips?

I mean things like: G418 concentration, cell concentration or any other special conditions.

 

Thank you,

Roman







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