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Protocol for isolating cells from Subcutaneous Tumors ?

tumor cell isolation

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#1 jpclamme

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Posted 11 September 2014 - 11:43 AM

Hi,

 

I was wondering if anyone had a good protocol to isolate cells from a subcutaneous tumor on mice.

 

Thank you

 

JP

 



#2 Rsm

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Posted 12 September 2014 - 03:13 AM

Mince and digest with 3mg/ml each Collagenase/Dispase in complete medium at 37C for at least 1h rotating. Better 3h. Filter 100um, spin and wash.


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