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I want to separate two proteins having mw of 16.8 and 16.78 kDa. how can i accom

protein electophoresis SDS-PAGE Proteins gel resolution

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#1 Vijaytailor

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Posted 10 September 2014 - 11:22 PM

Hi, I have a problem i want to separate two proteins having mass very nearby . hw to separate them?



#2 bob1

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Posted 11 September 2014 - 12:54 AM

As long a gel as you can get, 20 cm or more would be good. Run as long as you can.



#3 mdfenko

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Posted 11 September 2014 - 03:40 AM

that mass difference may not be separable with sds-page, although tris-tricine sds-page (with a long gel, as suggested by bob1, and maybe a shallow acrylamide gradient) would have the best chance.

 

you may be able to separate them by charge difference.

 

this can be done with isoelectric focusing page or with urea-page (i used urea-page to separate 20 kDa proteins with only a one or two phosphate difference).


Edited by mdfenko, 11 September 2014 - 03:41 AM.

talent does what it can
genius does what it must
i used to do what i got paid to do






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