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Can I treat my buffer with DEPC ?


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#1 Juanhijuan

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Posted 08 September 2014 - 08:10 AM

So I prepared a buffer for native protein extraction:

 

25 mM Tris

100 mM NaCl

10% glicerol

 

Can I treat this buffer with DEPC ?

 

 



#2 tkf

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Posted 08 September 2014 - 11:52 AM

No, Tris will hydrolyze DEPC. You can use PBS buffer instead.






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