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Perfusion of neonatal mouse?


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#1 Dbrainz

Dbrainz

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Posted 05 September 2014 - 08:27 AM

Hi, has anyone done an intracardiac perfusion on an infant mouse? I keep reading papers that just say to do it, but I am not sure how- what gauge needle would I use, what flow rate, how to get the skull off without mangling the brain (the answer to that one may be "be very careful...") 

 

Thanks, if you have done this, I would appreciate tips

 

-DB






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