Just wanted to share this as it may be relevant to saving money when shipping PCR products for sequencing.
Where we send the company website says they want overnight and in a cooler with dry ice (for crude PCR products in the PCR buffer solution). Theoretically and from a (inadvertent) past experience (involving 2 days at RT), they should be fine, for some time at least (if not having been open) at RT, because they’ve effectively been sterilized + any DNase enzymes degraded by being at 94-95 C.
I also did (gel pic attached) a check involving leaving two tubes at RT for ~48 hrs after they had been briefly opened (and thus potentially contaminated) at the bench in the course of running a gel. No difference was observed between them and the same product that was at -20 C the whole time.
I make this post because it might save some people a considerable amt on shipping to send it w/o cooling and possibly even 2nd day instead of overnight… as it would appear from my experience that virtually no degradation occurs (at least not within a few days).
P.S. Does anybody still do a final cool holding temp. after running a PCR? I would say it’s totally unnecessary (even if left overnight) and I’ve read it will speed up the machine wearing out, due in large part to the potential for water condensing and ruining the electronics.