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RT-PCR primer design and pseudogenes

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#1 sara.r



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Posted 30 August 2014 - 01:08 AM



I am designing some primers for detection of a gene using RT-PCR. The gene also has a pseudogene with high similarity to the transcript so my primers can amplify it and give the same amplicon size in PCR. As far as I know the processed pseudogenes are not transcribed so I was wondering whether DNase treatment of the RNA will be enough to avoid false amplification from the pseudogene.




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