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DMEM medium with HEPES and NaHCO3

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#1 sara.r



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Posted 29 August 2014 - 07:21 PM


I am going to order some DMEM medium and I noticed some medium contain NaHCO3 only while the others have both HEPES and NaHCO3. I read that the buffering system of NaHCO3 depend on CO2 but for HEPES it does not. But I do not know if is it necessary to have both buffers in medium. I am growing CHO and HEK cells in an incubator with  5% CO2, and so far I have not used medium with HEPES. So if I switch to HEPES/DMEM medium would it gives me better results?



#2 bob1


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Posted 30 August 2014 - 03:58 PM

Depends on how much Bicarb there is in the solution. Normally growth in a CO2 incubator will be fine with just HCO3-

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