I am preparing an expression vector to eventually express an ion channel in Hek cells. Right now I am about to ligate the channel DNA into the vector. I am only using EcoR1 (invitrogen) to digest the vector so i will need to dephosphorylate as well. My question is, can I run the restriction digestion and dephosphorylation (using NEB CIP) simultaneously in the same tube. If so, should i use both buffers? or one over the other?