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pBABE Comp. Cells


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#1 Triton

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Posted 07 July 2004 - 06:11 AM

I am subcloning some inserts into pBABE vector. I was told by another post-doc that I need to use a special competent cell line. Anyone have experience with this vector system?? Thanks.

-Steve

#2 labrat

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Posted 07 July 2004 - 08:23 AM

Better do a PubMed search to find out what bacteria others have used for transformation.

#3 ruzkhv

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Posted 17 July 2004 - 02:42 AM

:rolleyes: We are using subcloning efficiency chemicaly compotent bacteria 5DH alfa cells from invitrogene. works fine

#4 jadefalcon

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Posted 18 July 2004 - 11:23 PM

chemically competent DH5 alpha, as well as XL-1 blue, which are (our) standard strains work fine in our lab together with pBabe/Puro

Mike

Edited by jadefalcon, 18 July 2004 - 11:23 PM.

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