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PCR Products not run well in the GEL


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#1 duminda.rusl@gmail.com

duminda.rusl@gmail.com

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Posted 25 August 2014 - 01:31 AM

Dear All

 

Here I have a problem with my PCR product;  When I run my PCR product in the TEA Gel elctrophoresis, sharp bands were appeard but dusk like sturucture present through that bands.



#2 bob1

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Posted 25 August 2014 - 01:49 AM

Ethidium shadow is the most likely explanation - Ethidium bromide migrates in the opposite direction to the DNA, creating a darker area at the bottom of the gel.






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