Today I stripped 6 nitrocellulose blots using a DDT mixture in the oven. I proceeded to probe for tubulin. When I went to develop the blots, I noticed that some entire blots had robust signals across the board, other membranes had faint signals throughout.
Is it likely that this is because all 6 blots were stripped and incubated in a single container? How does stripping and incubating multiple in a single container generally effect results? I have done this same procedure for 4 blots and had robust signals for every blot. Any and all advice appreciated - please share your experiences with stripping or incubating multiple blots at one time.