I'm developing a purification protocol which includes a dialysis step, which is intended to separate a 90kD protein from a 1 kD inhibitor. I have read that it's a good idea to choose a MWCO for the membrane that is about halfway between the two sizes, i.e., about 50 kDa. Does this sound correct? Would a lower MWCO likely be effective as well? Thanks.
Edited by Artemis2007, 21 August 2014 - 09:46 AM.