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Making a fusion protein

primer desgin fusion protein foward primer reverse primer

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#1 Teapot_11



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Posted 20 August 2014 - 04:42 AM

Hi guys


At the moment I have a bit of a concern

I was wondering if any one had a spare mement and could explain to me

why do we need a string of AAAAAA, on our foward and reverse primers


I am cloning a fusion protein and am in the primer design phase

Is there a reason for the A's????|



Woudl appreciate any feedback


Yours Sincerely


#2 bob1


    Thelymitra pulchella

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Posted 20 August 2014 - 12:50 PM

Where on the primers do you need this string?


There are usually only three components you need in a primer that will make a product for digestion and subsequent cloning. You need a specific sequence targetedto the sequence you want to amplify, a restriction site, and some bases 5' of the restriction site to give the RE something to bind to. This last bit is possibly what you are referring to.

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