I have designed my forward and reverse primer with restriction site (BAMHI) at its 5 end using perl primer. i have run the PCR with an annealing temperature related to the melting temp of the primer complementary to the template. After run the gel, i got a single band around 500bp length but my target gene is 1200bp. after sequencing, i did not get the proper peaks for my product and i got only noise peaks. pl. anyone suggest whether changing the annealing temperature will end up in getting the targeted product or else this primer does not work out.