I am trying to isolate the nuclear protein from mesenchymal cell using the nuclear isolation kit from Active mottif and then using this for Co-IP. I have tried many times, but Iam not able to get the protein of interest. All I see is the IG bands on the gel. First Iam not sure if the nuclear proteins are being isolated. Can anyone help me in confiming this. If someone is doing the same procedure for mammanlian cells can you please help me out sorting this. Is this kit really good to isolate nuclear protein. Any suggestion would be helpful.