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Identification of Listeria monocytogenes

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#1 Dima

Dima

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Posted 18 August 2014 - 09:43 AM

Hello to all,

 

I have isolated Listeria monocytogenes from my food samples and I would like to identify the strains by PCR method.

 

your help is appreciated in these 3 points (if possible please):

 

1-I havent done this before or worked with L.Mono, but i have been reading that targeting the hly gene would identify the strains that i have. What other genes that could also be specific for L.Mono that i should target?

 

2- has anyone worked with the L.Mono identification by PCR and can share with me the protocol?? I need to order all reagents and primers that are essential to run this work. would be great when I get an advice.

 

3- My Listeria monocytogenes isolates are preserved in 30% glycerol (freezed). For DNA extraction,  Should I directly streak a loopful from the glycerol preserved stock onto the agar plate or I should better first dispense it in TSB for overnight at 37C then later streak it ?

 

I am not sure what is the best way to recover my Listeria glycerol stock and optimum subculturing methods to maintain my bacteria !

 

I hope to hear from you!

 

thanks in advance,

DIma

 

 

 

 



#2 Dima

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Posted 18 August 2014 - 11:38 AM

 i just found an answer on my earlier posts concerning the detection of virulent genes in Listeria mono.:

 

the "microseq listeria monocytogenes "detection kit..







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