Hello to all,
I have isolated Listeria monocytogenes from my food samples and I would like to identify the strains by PCR method.
your help is appreciated in these 3 points (if possible please):
1-I havent done this before or worked with L.Mono, but i have been reading that targeting the hly gene would identify the strains that i have. What other genes that could also be specific for L.Mono that i should target?
2- has anyone worked with the L.Mono identification by PCR and can share with me the protocol?? I need to order all reagents and primers that are essential to run this work. would be great when I get an advice.
3- My Listeria monocytogenes isolates are preserved in 30% glycerol (freezed). For DNA extraction, Should I directly streak a loopful from the glycerol preserved stock onto the agar plate or I should better first dispense it in TSB for overnight at 37C then later streak it ?
I am not sure what is the best way to recover my Listeria glycerol stock and optimum subculturing methods to maintain my bacteria !
I hope to hear from you!
thanks in advance,