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Pectinase Enzyme Assay

enzyme biochemistry Enzyme Assay Enzyme Kinetics

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#1 olij



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Posted 18 August 2014 - 05:58 AM

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?

#2 mdfenko


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Posted 20 August 2014 - 03:54 AM

you must give more information about your procedure. for instance, the procedure you wrote appears to be the enzyme-substrate reaction, not a blank. you would expect to see a high reading. how do you prepare your blanks? what are the readings?

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