I have a protocol for extracting DNA from ticks. The ticks that I am using are preserved in glycerol.
I wash them with 70% ethanol and then sterile water and then extract their DNA. I used 1 tick from the same vial each time.
The problem is at the end (after ethanol precipitation) I sometimes see the DNA pellet and sometimes I don't. Like I tried it 10 times. And I failed to get a DNA pellet 5 times. Any idea why this can happen? Is it that particular tick sample? Sometimes the tick have a missing leg, does that cause nuclease to get out and I don't get DNA?
I looking forward to your suggestions.