I have a dilemma if anyone was kind and knowledgeable enough.
I am cloning a N-terminal fusion protein, and I am designing my primers so that I have to remove the ATG codon of my gene of interest.
My question --> is it appropriate to remove the ATG from your protein and rely on the transcription of you fused protein within the vector?
I am little stumped on why you have to remove the ATG in the first place?
Would appreciate some ideas.
Yours Sincerely Liam Tegg