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PBMC cell stimulation?

PBMC Blood draw stimulation

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#1 Cyanide

Cyanide

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Posted 15 August 2014 - 05:23 AM

Hi All,

 

I was just wondering if anyone has done any work with looking at different blood draw methods for PBMC production and if this affects your results. I am wondering if blood collected by cannula in a vacutainer will lead to cell activation and poor cell viability in PBMCs and if it is better to collect blood via direct stab in syringes with anti-coagulant?

 

Thanks,

 

C.







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