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Chromatin Isolation Troubleshooting

Pellet Crosslinking ChIP

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#1 vp1987



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Posted 14 August 2014 - 01:18 AM

Hi guys!


I'm very new to ChIP, and I'm doing a pull down on chromatin from human PBMCs. I'm having trouble right in the very first step of PMBC isolation and subsequent washing with 1X PBS, where for some reason after I pellet my cells, they are sticking together and refusing to dissolve even after several minutes of flicking. I feel like this is affecting the rest of my experiment,


Any suggestions are welcome!


Thank you in advance!

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