I understand that for the bisubstrate enzyme catalyzed reactions involving formation of ternary complex, increasing the substrate-2 concentration also increases V-max and Km for substrate-1. I can understand the effect on V-max logically, but can anyone explain why should increasing substrate-2 should increase the Km for sustrate-1?
Submit your paper to J Biol Methods today!
Bisubstrate enzyme reaction kinetics
Started by rmbio, Aug 13 2014 12:33 AM
involving ternary comple
1 reply to this topic
#2
mdfenko
-
- Active Members
-
- 3,335 posts
an elder emeritus
213
Excellent
Excellent
Posted 13 August 2014 - 04:10 AM
the second substrate causes a conformational change in the enzyme which affects the active site for the first substrate.
talent does what it can
genius does what it must
i used to do what i got paid to do
