I understand that for the bisubstrate enzyme catalyzed reactions involving formation of ternary complex, increasing the substrate-2 concentration also increases V-max and Km for substrate-1. I can understand the effect on V-max logically, but can anyone explain why should increasing substrate-2 should increase the Km for sustrate-1?
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Bisubstrate enzyme reaction kinetics
Started by rmbio, Aug 13 2014 12:33 AM
involving ternary comple
1 reply to this topic
#2
mdfenko
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Posted 13 August 2014 - 04:10 AM
the second substrate causes a conformational change in the enzyme which affects the active site for the first substrate.
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