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smear in real-time pcr

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#1 sghafarpour



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Posted 09 August 2014 - 11:36 PM


I have smear in my real-time pcr result. i was extracted rna from ffpe samples. when i seted up, there was any problem but now all samples even non sample control. i did this test for three other target and they did not have smear or contamination. melt curve og test have two pick . in 84 or 87-89 . it should noted rna samples were treated with dnase.

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