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how to interpret or present elisa result for IgA in pig obtained from 120 saliva

elisa pig IgA

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#1 paul73

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Posted 09 August 2014 - 04:23 PM

Hi, I'm totally new in this type of experiment. Me and my colleague tried to develop a sandwich elisa for IgA quantification in pig saliva. we had 120 saliva samples and now we are in problem of presenting the IgA result of that large no. of samples in my paper. we tried to fit all samples in a graph (sample in x axis and optical density in y axis) but it looks awkward. It will be highly appreciated if you give your valuable suggestions to help me out.



#2 bob1

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Posted 10 August 2014 - 04:19 PM

If they are independent samples (not replicates) then you can't get around it.  You could perhaps group them by something (type of pig?, treatment?), and break them up into separate graphs that way



#3 paul73

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Posted 12 August 2014 - 04:31 PM

helo, bob thanks a lot for your suggestions. I feel to explain my experiment briefly to have more suggestions from you.  There were 3 control group and 3 test group of total 30 pigs. Samples were collected at different time frame and finally collected 120 samples. We received those samples from other experiments. We quantified IgA by two elisa: one is commercially available pig IgA elisa kit and other, our developed elisa. Now, I need to present and compare the results between two elisa. It is really complicated to me. 


Edited by paul73, 12 August 2014 - 04:33 PM.


#4 bob1

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Posted 13 August 2014 - 12:19 AM

In that case you don't have 120 samples, you have 6 samples with 30 replicates (which should hopefully be enough to give you statistical significance).  The idea would be to generate some statistics from your replicates (averag +/- standard deviation, box and whisker plot... talk to a statistician about suitable tests) and then graph those.







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