What do you think of a DNA sequencing facility that is using Peaktrace ( http://www.nucleics.com/peaktrace/ ) to generate "outstanding" chromatogram???
Usually chromatogram generate by ABI platform will show the peak become broader and resolution become lower due to limitation of the platform after 700 - 800bp eventhough most of the time it give reading up to 1000+ bp. Please attachment (SEQ1.ab1) for example.
However some facility are using Peaktrace to call "better" chromatogram which can show to end user that the chromatogram can show very nice, beautiful and high resolution peak even it is near 1000bp. Please attachment (SEQ2.ab1) for example.
Thus, there is voice saying that the facility which generate chromatogram using Peaktrace do a better job than the one that is using ABI KB basecaller.
What do you think about this??? The 2.ab1 file I attached are actually from same sequence trace data.
Hope to hear your opinions... Thanks!!!