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Screen for F plasmid in e. coli


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#1 Raygoza

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Posted 07 August 2014 - 08:25 AM

HI I've searched but can't find a reference on how to determine if an isolate of e coli has the F factor or not,i.e., F+, does anyone know how to do this?


Edited by Raygoza, 07 August 2014 - 08:25 AM.


#2 BioMiha

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Posted 07 August 2014 - 01:19 PM

You could just PCR a gene that's on the F+ plasmid and if it comes back positive, you'll know it's there. 






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