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Radioactive hybridisation


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#1 mishla

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Posted 05 July 2004 - 01:01 AM

[COLOR=red][FONT=Courier][SIZE=7][I]
Why is that most of the labs use alpha P32 labelled dCTP for labelling?

Is ther any specific reason for using this label?

Plz reply!

Edited by mishla, 05 July 2004 - 01:15 AM.


#2 Yasmine

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Posted 05 July 2004 - 03:26 AM

Mishla,
People usually use dCTP to label all along a DNA strand as it is normally alpha labelled and dATP just to label at the 5' Phosphate group as it usually is gamma labelled.
I hope it helps
Yasmine.




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