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Insulin - stock solution?

Cell culturing insulin

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#1 Lee24

Lee24

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Posted 04 August 2014 - 03:27 AM

Hi,

 

I am new to cell culturing and am hoping that some of you could help me with a few simple questions please? I will be working with L6 myoblast cells and also primary skeletal muscle myoblast cells. 

 

I have just bought some insulin (10 mg/ml) from Sigma (http://www.sigmaaldr...ng=en&region=GB) and would like to dilute this down in PBS and keep stocks in small aliquots in the freezer. 

 

My questions are;

 

1. What uM concentration would be appropriate for to store?

2. How long can I store it for?

3. Would storing them at -20C be OK, or would it require -80C?

 

Any help on this matter would be hugely appreciated. 

 

Lee

 

 

 

 

 

 







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