I need your help again. I'm looking for a software / online tool that is able to look through a certain sequence of cDNA and can find "new" restriction sites by changing a single codon so that the initial amino acid is not changed.
I mean it like this:
Starting sequence: GTTGACATGCGAATGCG
Software will suggest: GTCGACATGCGAATGCG
GTT encodes for valine, changing GTT to GTC does not alter the amino acid sequence but gives rise to a SalI restriction site.
I've looked throughout the internet, downloaded a lot of "cloning software" but I wasn't able to find any software / tool that can do this. So if anyone of you knows something that works well (and is free of charge) I'd be very thankful for your input.