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Grow Acetobacter

Acetobacter

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#1 Adriana Reis

Adriana Reis

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Posted Today, 05:37 AM

Hello Everyone

This is my first post.

 

I am having quite the problematic bug here.

 

I have been trying with no success to grow Acetobacter pasteurianus.

 

First I have tried with this broth 

  • 500ml 

7.5g of malt extract

2.5g of yeast extract

add 470ml of water

and autoclave

 

then add 30ml of ethanol 50% filtered

when it left the autoclave broth was filled with a precipitate

 

grow at 26C

but had no growth

 

  • tried this one instead 1L

peptone 3g

yeast extract 5g

mannitol 25g

grow at 28C with no luck

 

tried after to grow it at 26C but also with no luck

 

  • I am trying to get CaCO3 so I could try a different one

1L

glucose 100g

yeast 10g

CaCO3 20g

pH 6.8

 

Anyone can suggest something to help me with this?

 

I am probably doing something wrong!!!

 

 

Thank you

Adriana Reis

 

 



#2 Phil Geis

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Posted Today, 09:52 AM

Try lowering p H to < 5




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