This is my first post.
I am having quite the problematic bug here.
I have been trying with no success to grow Acetobacter pasteurianus.
First I have tried with this broth
7.5g of malt extract
2.5g of yeast extract
add 470ml of water
then add 30ml of ethanol 50% filtered
when it left the autoclave broth was filled with a precipitate
grow at 26C
but had no growth
- tried this one instead 1L
yeast extract 5g
grow at 28C with no luck
tried after to grow it at 26C but also with no luck
- I am trying to get CaCO3 so I could try a different one
Anyone can suggest something to help me with this?
I am probably doing something wrong!!!