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panc21 cell line look sick

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#1 balloon14



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Posted 30 July 2014 - 02:35 AM

hi, i am growing PANc21 pancreatic cancer cell line. After 2, 3 passages the cells started to look unhealthy and sick. It started to detach from the flask and are not reaching the confluence. There is no bacterial or fungal contamination. I am using High glucose DMEM media. If anybody know the reason and its treatment plz let me know.

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