Yes, I can see your point.
According to the seller of the system it should not be such a problem. I just noticed it the last few weeks that the system is not that bulletproof!
I also sequenced the gene itself and it still is the original fragment! Perhaps in some colonies it is indeed a problem with mutations.
Your system, I remember it from a while ago, seems nice, but for the work I am doing its too much work I am afraid.
And the biggest problem is pretty much the fact that the genes I have to use are given to me in a gateway plasmid.
So I am pretty much forced to use it.
The PCR amplification and digesting , restriction free cloning as I know it, is indeed a very usefull system. I pretty much use it all the time when I can!
Makes me wonder why so many people still keep getting stuck working with restriction enzymes and ligations and so on.