I have some oligos to anneal to clone into a plasmid.
The problem is however that one of the people that also uses these dissolved them in the wrong buffer.
We normally use an annealing buffer of 1mM EDTA, 50mM NaCl and 10mM Tris however the buffer they used to dissolved the oligos contained the wrong EDTA concentration: 25 times the amount : so 25mM rather than 1mM.
Anyone an idea if this would be a problem if I tried to make the oligos to anneal?
I could add some more NaCl and Tris to dilute the EDTA , however I would have to dilute the oligos a lot than and I think they would not anneal anymore if I dilute it too a concentration that is rather low.
Now I have a 100micromolar concentration of oligos, diluting this 25 times seems a bit too much since I would end up with just 4 micro molar of the oligos.
Edited by lyok, 29 July 2014 - 10:18 AM.