I have a silly confusion.I wanted to know whether a restriction enzyme can recognize a incompatible site with compatible sticky endand cut it?
For e.g, SpeI and XbaI can generate compatible sticky ends but a slightly different recognition sites
For XbaI recognition site is TCTAGA and it cuts between T and C to generate T CTAGA
AGATCT AGATC T
For SpeI recognition site is ACTAGT and it cuts between A and C to generate A CTAGT
TGATCA TGATC A