The Cloning Gods have definitely not been with me lately!
Everyone in the lab has been having issues cloning too :/ I suspect contamination but how on earth do we go about cleaning things up? I've never had this issue before.
I have attached a gel from yesterday where lanes 2, 5-13 contain all sorts of unexplained bands (this is a NotI digest). Colonies were grown on LB Amp plates. I used fresh commercially available competent cells.
Any ideas on how to make this go away?
Thanks in advance!
xxx me and the rest of the lab