I am trying to set up co-culture system with 3T3-L1 and C2C12.
I never had previous experience about co-culture. Sure I have some experience for jsut regular cell culture.
Could anyone can give some general information how to set up co-culture system and what kinds of tools (dishes and where?) I should purchase.
Could anyone who has this two cell types coculture experience give a protocol??
Should I use the same media for both cells? or each their media in co culture?
THANKS IN ADVANCE.